Characterization and Determination of Major Bioactive Acids in Camel Urine Using Gas Chromatography Mass-spectrometry
نویسندگان
چکیده
The major acidic and phenolic biogenic materials in adult-camel urine were characterized using gas chromatography-mass spectrometry. The adult-camel urine sample was treated with glucuronidase/ arylsulfatase enzyme followed by extraction on Sep-Pak® C8 column. The water soluble compounds, including urea and creatinine, were washed out using water containing 0.4% trifluoroacetic acid. Ethyl acetate:diethylether, 1:1 v/v, was used as extraction solvent. The extraction residue was reprivatized with N-methyl-N-(trimethylsilyl) trifluoroacetamide followed by gas chromatography-mass spectrometry analysis. The average concentrations of, phenol, p-cresol, salicylic acid, cinnamic acid, azelaic acid and benzoic acid were 4.0, 107.2, 42.7, 3.2, 68.6 and 490.6 mg/100 ml, respectively. The calibration range of each of these compounds was spanning the range of 1.0 to 50 ng/μl. The extraction recovery of all studied compounds was 100±0.8%. A standard solution mixture containing the major acidic and phenolic compounds was prepared to contain the same corresponding concentration in adult-camel urine. Both, adult-camel urine and prepared standard mixture were active against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus flavus (fungus) and Candida albicans (fungus) after 72 h of incubation.
منابع مشابه
Relative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation
The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites...
متن کاملRelative Determination Approach to the Metabolites of Protoberberine Alkaloids in Rat Urine by Liquid Chromatography Tandem Mass Spectrometry for the Comparative Studies on Rhizome coptidis and Zuojinwan Preparation
The lack of authentic standards has limited the quantitative analysis of herbal drugs in biological samples. The present work demonstrated a practicable strategy for the assay of herbs and their metabolites independent of authentic standards. A liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) method for the qualitative and quantitative determination of the metabolites...
متن کاملDevelopment and Validation of a GC-FID Method for Diagnosis of Methylmalonic Acidemia
Background: Urinary organic acids are water-soluble intermediates and end products of the metabolism of amino acids, carbohydrates, lipids, and a number of other metabolic processes. In the hereditary diseases known as organic acidurias, an enzyme or co-factor defect in a metabolic pathway leads to the accumulation and increased excretion of one or more of these acidic metabolites. Gas chromato...
متن کاملCharacterization of Bioactive Compounds in Tunisian Bitter Orange (Citrus aurantium L.) Peel and Juice and Determination of Their Antioxidant Activities
Citrus aurantium peel and juice aroma compounds were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), whereas phenolic compounds analysis was performed by reversed-phase high-performance liquid chromatography (RP-HPLC). Limonene was the major volatile compound of bitter orange peel (90.25%) and juice (91.61%). HPLC analysis of bitter orange peel and juic...
متن کاملSimultaneous Determination of 13 Priority Polycyclic Aromatic Hydrocarbons in Tehran’s Tap Water and Water for Injection Samples Using Dispersive Liquid-Liquid Micro Extraction Method and Gas Chromatography-Mass Spectrometry
Abstract Polycyclic aromatic hydrocarbons (PAHs) are classified as persistent and carcinogenic organic pollutants. PAHs contamination has been reported in water. Many of relevant regulatory bodies such as EU and EPA have regulated the limit levels for PAHs in drinking water. In this study, 13 priority polycyclic aromatic hydrocarbons (PAHs) were determined in tap water samples of Tehran and wat...
متن کامل